Kugwiritsa ntchito ultrafiltration mu kulekanitsa mapuloteni ndi kuyeretsa

Ukadaulo wa Ultrafiltration ndiukadaulo watsopano komanso wopambana kwambiri wolekanitsa.Zili ndi makhalidwe a njira yosavuta, phindu lalikulu lachuma, palibe kusintha kwa gawo, koyeneka yolekanitsa yaikulu, kupulumutsa mphamvu, kugwiritsira ntchito mphamvu, kusakhala ndi kuipitsidwa kwachiwiri, kugwira ntchito mosalekeza kutentha kwa firiji ndi zina zotero.Masiku ano, manejala Yang yemwe waku Beijing adafunsa za zida zathu zoyeretsera mapuloteni ndikulumikizana mwatsatanetsatane ndiukadaulo wathu.Tsopano, mkonzi wa gulu la Shandong Bona adzayambitsa kugwiritsa ntchito ultrafiltration mu kulekanitsa mapuloteni ndi kuyeretsa.

1. Pakuti mapuloteni desalination, dealcoholization ndi ndende
Ntchito yofunika kwambiri ya ultrafiltration mu kuyeretsa mapuloteni ndi desalting ndi ndende.Ultrafiltration njira desalination ndi ndende amakhala ndi lalikulu mtanda voliyumu, yochepa ntchito nthawi ndi mkulu dzuwa kuchira mapuloteni.Njira yachikhalidwe yochotsera steric exclusion chromatography kuchotsa zinthu zosiyanasiyana kuchokera ku mapuloteni yasinthidwa ndiukadaulo wamakono wa ultrafiltration, womwe wakhala ukadaulo waukulu wa protein desalination, dealcoholization ndi ndende lero.M'zaka zaposachedwa, ukadaulo wa ultrafiltration wagwiritsidwa ntchito kwambiri pochotsa mchere komanso kubwezeretsanso mapuloteni opatsa thanzi mu cheese whey ndi soya whey.Lactose ndi mchere ndi zigawo zina mu mapuloteni, komanso zofunika zenizeni kukwaniritsa bwino desalting, de-alcoholization ndi ndende ya mapuloteni.Kugwiritsa ntchito ukadaulo wa ultrafiltration kumathanso kuyang'ana kwambiri ma serospecies immunoglobulins kuti akwaniritse kufunikira kwenikweni kwa zokolola zama protein.

2. Kugawanika kwa mapuloteni
Kugawa kwa mapuloteni kumatanthawuza njira yolekanitsa chigawo chilichonse cha puloteni ndi gawo malinga ndi kusiyana kwa thupi ndi mankhwala (monga molekyulu yolemera, isoelectric point, hydrophobicity, etc.) ya chigawo chilichonse cha mapuloteni mumadzimadzi amadzimadzi.Gel chromatography ndi imodzi mwa njira zomwe zimagwiritsidwa ntchito kwambiri pogawa ma biological macromolecules (makamaka mapuloteni).Poyerekeza ndi chromatography yachikhalidwe, ukadaulo wolekanitsa wa ultrafiltration uli ndi chiyembekezo chabwino chogwiritsidwa ntchito pakugawa ndi kupanga mapuloteni ndi ma enzymes omwe ali ndi phindu pazachuma chifukwa chotsika mtengo komanso kukulitsa kosavuta.Dzira loyera ndiye zotsika mtengo zopangira lysozyme ndi ovalbumin.Posachedwapa, ultrafiltration nthawi zambiri amagwiritsidwa ntchito kulekanitsa ovalbumin ndi lysozyme kuchokera ku dzira loyera.

3. Kuchotsa Endotoxin
Kuchotsa endotoxin ndi imodzi mwazinthu zazikulu zogwiritsira ntchito ukadaulo wa ultrafiltration pakuyeretsa mapuloteni.Kapangidwe ka endotoxin ndizovuta kwambiri.M'kati mwa ntchito zothandiza, chifukwa mapuloteni mankhwala opangidwa ndi prokaryotic mawu dongosolo n'zosavuta kusakaniza ndi endotoxin opangidwa ndi bakiteriya selo khoma kusweka, ndi endotoxin, amatchedwanso pyrogen, ndi mtundu wa lipopolysaccharide.Mukalowa m'thupi la munthu, zimatha kuyambitsa kutentha thupi, kusokonezeka kwa microcirculation, kugwedezeka kwa endotoxic ndi zizindikiro zina.Pofuna kuteteza thanzi la munthu, ndikofunikira kugwiritsa ntchito ukadaulo wa ultrafiltration mokwanira kuti muchotse endotoxins.

Ngakhale teknoloji ya ultrafiltration imagwiritsidwa ntchito kwambiri pakulekanitsa ndi kuyeretsa mapuloteni, imakhalanso ndi malire.Ngati kulemera kwa maselo azinthu ziwiri zomwe ziyenera kulekanitsidwa ndi zosakwana 5 nthawi, sizingalekanitsidwe ndi ultrafiltration.Ngati kulemera kwa mamolekyu a chinthucho kuli kochepa kuposa 3kD, sikungalowetsedwe kwambiri ndi ultrafiltration, chifukwa ultrafiltration nthawi zambiri imachitika pa mlingo wocheperako wa molekyulu wa nembanemba pa 1000 NWML.

Ndi chitukuko chosalekeza chamakampani opanga bioengineering, zofunikira zapamwamba zimayikidwa patsogolo pakulekanitsa kwapansi ndi ukadaulo woyeretsa.Njira zachikhalidwe za ndende ya vacuum, zosungunulira m'zigawo, dialysis, centrifugation, mpweya ndi kuchotsa pyrogen sizikupezekanso kuti zikwaniritse zosowa za kupanga.Ukadaulo wa Ultrafiltration uyenera kugwiritsidwa ntchito mochulukirachulukira chifukwa cha zabwino zake pakulekanitsa kwa mapuloteni.